Sumoylation-Specific Antibody Introduction

Sumoylation is a form of post translational modification (PTM) of proteins similar to ubiquitination modifications. It can modify a variety of gene transcription factors, messenger molecules, and viral proteins. Studies have shown that sumoylation underlies many physiological processes and plays an important role in life activities. However, because sumoylation has not been discovered for a long time, the understanding of sumoylation is still far from adequate. Therefore, more efforts need to be devoted to the study of sumoylation. Based on our phage display platform and hybridoma technology platform, Creative Biolabs provides comprehensive sumoylation-specific antibody development services to facilitate sumoylation research.

Sumoylation Modification

Small ubiquitin-like modifier (SUMO) proteins are a series of conserved eukaryotic protein modifiers consisting of approximately 100 amino acids. The conjugation of SUMO to the substrate lysine is accomplished by SUMO E1, E2, and E3 enzymes. Once conjugated to a substrate, SUMO can exert a variety of effects. These effects include altering the interaction of the substrate with DNA, RNA, or other proteins, changing conformation or enzyme activity, and modulating other modifications. Sumoylation is a reversible process that is catalyzed by a small number of modifying enzymes but dynamically regulates thousands of target proteins. Sumoylation is catalyzed by SUMO-specific E1, E2, and E3 enzymes, while de-sumoylation is regulated by the SENPs family. SUMO-1, SUMO-2, and SUMO-3 are the major SUMO proteins. Sumoylation and desumoylation are critical for centromeric structures and functions. Centromeres contain specialized histones and silenced chromatin, supporting the assembly of microtubule-attached kinetochores during mitosis. In a variety of organisms, SUMO pathway enzymes are enriched at centrioles and kinetochores, and the sumoylation of many proteins synergistically regulates the structure and function of centrioles. Overall, sumoylation primarily regulates nuclear processes, including gene expression, DNA damage response, RNA processing, cell cycle progression, and protein homeostasis.

Sumoylation-Specific Antibody

As with most PTM-specific antibodies, sumoylation-specific antibodies are generally difficult to develop, and there are not many commercially available antibodies. Antibodies are essential tools in protein sumoylation studies, which are crucial for a deeper understanding of sumoylation. Therefore, in sumoylation studies, it is often necessary to custom produce appropriate antibodies for subsequent studies.

Before preparing the immunogen, we need to identify the sites for sumoylation modification according to our research needs in combination with the references. This is crucial for the success or failure of the whole experiment. Since sumoylation is a reversible process, we need to strictly follow the extraction requirements when preparing the immunogen, ensuring low temperature and extraction speed to maintain the activity of the extracted protein. After generating sumoylation-specific antibodies, we recommend identifying the sensitivity and specificity of the antibodies through peptide competition assays, western blotting, dot blotting, indirect immunofluorescence, and other methods.

Creative Biolabs has a wealth of knowledge and experience in PTM specific antibody discovery. We would be happy to discuss our knowledge and experience in sumoylation-specific antibody development with you.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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